美国Electron Microscopy Sciences公司提供的几百种生物学研究领域的相关设备和配件及化学制剂，满足组织、细胞研究等生物领域的应用。

Acetamido to Butyl

4-Acetamido-4\'-Isothiocyanostilbene-2,2\'-Disulfonic Acid (SITS), Sodium Salt

C₁₇H₁₂N₂O₇S₃Na₂ F.W. 498.50 CAS #5-1023-76-8 SITS has been used as a vital stain, as a label for immunostaining, and as a neuronal tracer. SITS is also a non-penetrating fluorescent marker for erythrocyte membranes which acts as a potent inhibitor of anion transport. For more information related to the preparation and characterization of SITS, please refer to: Jakobsen, P. and Horobin, Richard W.(1989). Stain Technology, Vol.64, No.6 pg.301-313.

Acetate Buffer

Please seelistinginPrepared Buffers.

Acetic Acid, Glacial, Reagent A.C.S

C2H4O2 F.W. 60.05 CAS 64-19-7

Acetic Acid, Aqueous Solution

Prepared from acetic acid glacial with de-ionized water

RT	10042-02	Acetic Acid, 0.2% (v/v) Solution		500 ml	18.00	Add to Cart
RT	10042-05	Acetic Acid, 0.5% (v/v) Solution		500 ml	19.00	Add to Cart
RT	10042-10	Acetic Acid, 1.0% (v/v) Solution		500 ml	21.00	Add to Cart
RT	10042-50	Acetic Acid, 5.0% (v/v) Solution		500 ml	29.00	Add to Cart

Acetone, Reagent, A.C.S.

CH₃COCH₃ F.W. 58.08 CAS #67-64-1 A.C.S. Specifications:

Acetone, Glass Distilled CAS #67-64-1

U.V. Absorption - 1cm cell vs. H₂O

Acetone: Alcohol

Please seelistinginReagents for Histology: Fixing, Dehydrating, Clearing and Staining.

Acetonitrile, Reagent, A.C.S.

CH₃CN FW 41.05 CAS #75-05-8 A non-carcinogenic substitute for Propylene Oxide, used in tissueprocessing for TEM. Good for infiltration with EMbed-812 or other Epon-812 substitutes. Tarnowski, sB.I. et al, EMSA proceedings, Detroit, MI page 38 (1984)

TechnicalTip

Use of Acetonitrile

Acid Alcohols

An acidified alcohol solution for the differentiation of regressive stains.

RT	26071-01	Acid Alcohol, 0.5% HCl in 70% Alcohol		1 L	36.00	Add to Cart
RT	26071-04	Acid Alcohol, 0.5% HCl in 70% Alcohol	4x1 L	128.00	Add to Cart
RT	26072-01	Acid Alcohol, 1% HCl in 70% Alcohol		1 L	32.00	Add to Cart
RT	26072-04	Acid Alcohol, 1% HCl in 70% Alcohol	4x1 L	112.00	Add to Cart
RT	26073-01	Acid Alcohol, 1% HCl in 95% Alcohol		1 L	42.00	Add to Cart
RT	26073-04	Acid Alcohol, 1% HCl in 95% Alcohol	4x1 L	142.00	Add to Cart

Acid Fuchsin, Certified, C.N. #DcR-8

(Rubin S, Fuchsin S, Fuchsin Acid, Acid Magenta, Acid Violet 19) C₂₀H₁₇N₃Na₂O₉S₃ F.W. 585.54 CAS #3244-88-0 Solubility: 0.053% Water; 1.21% Alcohol

Acridine Orange

(Basic Orange 14) C₁₇H₂ON₃Cl F.W. 301.82 CAS #65-61-2

Ultrastructural probes on DNA templates within human bone marrow and lymph node cells. Frenstar, J., Adv. Cell Mol. Biol., 3, 1(1974). Used to detect changes in DNA template activity within cells. Frenster, J. Cell Nucl., 1, 565 (1974) Fluorescent dye for cytochemistry. J. Histochem. Cytochem., 31, 737 ((1983) J. Lab. Med., 15 (3),180, (1984)

Adhesives and Mountants

Please see theAdhesive and Mountants section.

Agar, Powder, Bacteriological Grade

(C₁₂H₁₈O₉)x F.W. 3000-9000 CAS #9002-18-0

Specifications:

Agarose I™

(C₆H₁₀O₅·C₆H₈O₄)_n CAS # 9012-36-6

AgaroseI™ is standard melting/gelling agarose, suitable for routine nucleic acid analytical/preservative applications. Agarose I™ has a low EEO for shorterelectrophoresis runs without compromising resolution. Excellent for blotting technique and general manipulations.

Biotechnology Grade:

™ Trademark of Amresco®

TechnicalDataSheet

Agarose for the Separation of Biological Molecules by Gel Electrophoresis

Agarose

Agarose SFR™

(C₆H₁₀O₅·C₆H₈O₄)_nCAS #9012-36-6

Agarose SFR™ is a high resolution sieving agarose with unsurpassed clarity. DNA bands differing in size by 2% can be resolved in the range of 200 – 1,000 bp. This agarose is suitable for the analysis of AFLP\'s (Amplified Fragment Length Polymorphisms), STR\'s (Short Tandem Repeats) and tetranucleotide repeats. The low melting point of agarose SFR™ makes it an excellent medium for analytical and preparative electrophoresis.

Biotechnology Grade

™ trademark of Amresco®

Agarose LE Powder

Features

EMS Agarose LE is a highly purified agarose, suitable for a variety of molecular biology applications. It is refined using an advanced process that excludes the use of organic solvents, yielding a cleaner end-product with a significantly reduced environmental impact. EMS Agarose LE can be used for analyses of proteins and nucleic acids of various sizes (150 bp to 6 kbp).

It\'s low EEO (≤ 0.13 -mr) promotes increased electrophoretic mobility, yielding improved resolution and shorter run times. This also allows macromolecules and larger particles (subcellular fragments, viruses, etc.) to migrate more freely through the gel matrix. The consistently low EEO also provides a reduction in band distortion (caused by counterflow) that can result from the presence of excessive sulfate-rich negative ions.

EMS Agarose LE is widely used for nucleic acid electrophoresis (analytical and preparative), protein electrophoresis (including radial immunodiffusion) and various blotting protocols. It is easily soluble, free of nucleases, and easy to use. It is highly transparent (forms a clear, colorless solution at 1g:100ml H₂O), and exhibits exceptionally low absorption of chemical staining agents. Pore size can be adjusted by simple modifications to the concentration ratio.

Formulated for high gel strength and integrity, EMS Agarose LE exhibits exceptional thermal stability and mechanical resistance, ensuring safe, easy handling, regardless of whether a denaturing agent has been added.

Specifications

Ordering Information

EZ Pack™ Agarose Tablets

Features

Researchers trust EMS Agarose LE to meet their electrophoretic needs for a variety of applications. Now the same EMS agarose is available in a convenient, no mess tablet.

EMS Agarose LE is refined in an advanced process that excludes the use of organic solvents. The result is a cleaner end product with significantly reduced environmental impact. The EMS agarose can be used for analyses of nucleic acids from 150 bp to 6 kbp, protein electrophoresis and various blotting protocols.

The low EEO of the EMS agarose promotes increased electrophoretic mobility, yielding improved resolution and shorter run times. This also allows macromolecules and larger particles (subcellular fragments, viruses, etc.) to migrate more freely through the gel matrix. The consistently low EEO reduces band distortion (caused by counterflow) that can result from the presence of excessive sulfate-rich negative ions.

The quick dissolving EMS EZ Pack™ tablet contains 0.5g (500 mg) of EMS Agarose LE, eliminating the hands-on time and inaccuracies normally associated with weighing. Use of the tablet is not only convenient and cleaner, it also provides better consistency and reproducibilty gel to gel. Gel preparation is simple – add the desired number of tablets to electrophoresis buffer, allow to sit for two minutes and then heat and pour as usual. The resulting gels are highly transparent, have exceptional thermal stability (ensuring safe and easy handling) and exhibit exceptionally low absorption of chemical staining agents.

EMS EZ Pack™ Agarose Tablets are supplied in convenient blister packs for safe, clean dispensing.

Specifications

EZ Chart

Ordering Information

Alcian Blue 8GX, C.N. #AnAn-6 (Ingrain Blue I)

C₅₆H₆₈N₁₆S₄Cu F.W. 1298.88 CAS #12040-44-7 Dye Content: Approx. 50% Solubility: Water-soluble

Used as a fixative: 0.5-2% Alcian Blue in Glutaraldehyde fixative enhances the preservation and constrast of the cell coat and intercellular substances. Used as a stain: For staining cell surface materials and cartilage components. Schofield, et al., Histochem J. 7, 139,1975 Used as a wetting agent: Sommer, J. R (1977). To cationize glass. J.Cell Bio. 75, 245a. Alcian Blue and Ruthenium Red were used to study cartilage ultrastructure. Thyberg, J., Lohmander, S., and Friberg, U., J. Ultrastruct. Res., 45, 407 (1973).

TechnicalTip

Purification of Alcian Blue

Congo Red Solution

Alizarin Red S, Certified, C.N. #DcAr-5

(Alizarin Camine, Mordant Red 3) C₁₄H₇O₇SNa^.H₂O F.W. 360.28 CAS #130-22-3 Solubility: 7.69% Water; 0.1% Alcohol.

Certified for Staining Bone. Stain for nervous tissue in small invertebrates, and for sections of nervous tissue. Lee, \"The Microtomist\'s Vade-Mecum\", 10th Ed., The Blakiston Co., Phila., 1937; Dawson, Stain Tech., 1, 123 (1926); Lundvall, Anat. Anz., 27, 521 (1905); 137. Use to differentiate bone from cartilage in mammalian embryos; used with Toluidine Blue. William, Stain Tech., 16, 23 (1941)

Aluminum Oxide 90 Basic

Al₂O₃F.W. 101.96 CAS 1344-28-1

Aluminum Oxide 90 Basic (63-200 Micron)for use withTechnovit® 9100.

Amphibian Ringer Solution

Ingredients: NaCl; KCl; CaCl₂·6H₂O and Distilled water.

Ammonium Hydroxide, 28% Reagent, A.C.S.

NH₄OH F.W. 35.05 CAS #1336-21-6 Specific Gravity: 0.91 Assay 28-30%

Specifications:

Ammonium Molybdate, Reagent, 99%

(Molybdic acid, ammonium salt) (NH₄)₆MO₇O₂₄·4H₂O F.W. 1235.86 CAS #12027-67-7

Used in the negative staining of viruses for TEM. Modifications caused by uncoupling agents. Muscatello, U., et al, J. Ultrastruct., Res., 52, 2 (1975)

Amyl Acetate, Reagent, A.C.S.

CH₃COOC₅H₁₁ FW 130.18 CAS #628-63-7 Boiling Range: 140.0°C-150.0°C Specific Gravity @ 25°C: 0.870-0.875A.C.S. Specifications:

Amyl Acetate, Purified, Glass Distilled

CAS #628-63-7

Aniline Blue, Water Soluble, C.N. #DcK-11

(Acid Blue 22; China Blue; Cotton Blue; Marine Blue V; Soluble Blue 3M, 2R; Water Blue) C₃₂H₂₅N₃O₉S₃Na₂ F.W. 737.74 CAS #28631-66-5

Solubility: 50% Water; 0.0% Alcohol; 4.5% Cellosolve; 7.8% Glycol; 0.0% Xylene Used in connective tissue staining procedures to stain collagen. Used in differential staining. Snodgress el al., Lab Invest. 26:329, 1972.

Antibody Diluent

Buffer for diluting your primary and secondary antibodies, especially if they were stored for a while, even at -20 in glycerol, or in refrigerator. Nonspecific binding of the antibodies, negative effects of disturbing substances and low or medium affinity cross-reactivities of the antibodies will be minimized, making your result more reliable. Excellent for IHC (frozen and formalin sections), flow cytometry on fixed cells, Western Blot and other immune assays.

When used in pathology, in also greatly reduces non-specific reactivity of human serum components and immunoglobulins in tissue, vessels and cells with mouse antibodies used on section.

For especially \"trouble\"-giving antibodies, as well as for in situ PCR applications, this dilutent may also be used as a washing buffer, preventing secondary binding of your analystes during washing.

4-8°C	62713	Antibody Diluent		50 ml	168.00	Add to Cart
4-8°C	62714	Antibody Diluent	125 ml	371.00	Add to Cart
4-8°C	62715	Antibody Diluent	500 ml	1,395.00	Add to Cart
62713-01	Antibody Diluent for Frozen Sections
4-8°C	62714-01	Antibody Diluent for Frozen Sections	125 ml	371.00	Add to Cart
4-8°C	62715-01	Antibody Diluent for Frozen Sections	500 ml	1,395.00	Add to Cart

Araldite Resin

W.P.E. number included on each bottle.

RT	10900	Araldite 502; CAS #84-74-2, Specific Gravity: 1.13		450 ml	18.00	Add to Cart
RT	10910	Araldite 506; CAS #25068-38-6, Specific Gravity: 1.13		450 ml	18.00	Add to Cart
RT	10920	Araldite 6005; CAS #3101-60-8, Specific Gravity: 1.16		450 ml	18.00	Add to Cart
RT	10930	Araldite 507; CAS #26447-14-3, Specific Gravity: 1.14		450 ml	18.00	Add to Cart

Araldite Embedding Kits

Please seelistinginEmbedding Kits.

RT	13900	Araldite 502 Kit consists of:	Kit	43.00	Add to Cart
450 ml Araldite 502
450 ml DDSA; Specially Distilled
*50 ml DMP-30

RT	13920	Araldite 6005 consists of:	Kit	46.00	Add to Cart
450 ml Araldite 6005
450 ml DDSA; Specially Distilled
100 ml BDMA
225 ml DBP

RT	13940	Araldite-EMbed (Mollenhauer\'s Kit):	Kit	64.00	Add to Cart
450 ml EMbed-812
450 ml Araldite 502
450 ml DDSA; Specially Distilled
*50 ml DMP-30

TechnicalTips

For Araldite RD-1 seeBGE. For Araldite RD-2, seeBDE

Auramine O, Certified, C.N. #DcAu-4

(Pyoctanunum aureum; Basic Yellow 2; Pyoktanin Yellow; Canary Yellow) C₁₇H₂₁N₃HCl·H₂O F.W. 321.86 CAS # 2465-27-2 Solubility: 1.0% Water; 4.0% Alcohol; 1.2% Cellosolve; 1.75% Glycol; 0.05% Xylene

Azocarmine G

(Acid red 101; Basalan Carmine GE; Rosinduline) C₂₈H₁₇N₃Na₂O₆S₂ F.W. 601.57 CAS #25641-18-3 Solubility: 1.0% Water; 0.1% Alcohol; 1.75% Cellosolve; 4.5% Glycol; 0.01% Xylene

Azur A (Methylene Azur A)

C₁₄H₁₄ClN₃S F.W. 291.80 CAS #531-53-3 Solubility: Water-soluble; Alcohol-soluble

Azur B (Azur I; Methylene Azur B)

C₁₅H₁₆N₃SBr FW 350.29 CAS #531-55-5

Solubility: Water-soluble; Alcohol-slightly soluble. An important component of Giemsa\'s stain for blood protozoa. Used for staining semi-thin sections of plant tissue. Hoefert, L.L. (1968). Polychromatic stains for thin sections of Beta embedded in epoxy resin. Stain Tech. 43:145

Azur II

(Azur B/Methylene Blue 1:1) CAS #37247-10-2

Azur II Eosin

(Azur B/Methylene Blue/Eosin 1:1:1) CAS #53092-85-6

Azur Mixture Solution

Please seeGiemsa Solution.

Basic Fuchsin, Certified, C.N. #DcFb-26

(Rasaniline Chloride; Magenta; Basic Violet 14) C₂₀H₁₉N₃^.HCl F.W. 337.86 CAS #632-99-5

Berkowitz et al., (1968). Selective staining of nervous tissue for light microscopy following preparation for electron microscopy. J. Histochem. Cytochem. 16:808. Sato, T., and Shamoto, M (1973). A simple rapid polychrome stain for epoxy-embedded tissue. Stain Technol. 48:223.

Basic Green 4, Certified, C.N. #DcMg-11

(Malachite Green Oxalate; Diamond Green B; BX or P Extra; Light Green N, Solid Green O; Victoria Green B or WB) 2(C₂₃H₂₅N₂)^.2(C₂H₂O₄)^.C₂O₄ F.W. 927.03 CAS #2437-29-8 Solubility: 10.0% Water; 8.5% Alcohol; 5.5% Cellosolve; 7.0% Glycol; 0.0% Xylene. Used as a bacteria stain and a counterstain for botanical material.

Benzoin, U.V. Catalyst

C₆H₅CH(OH)COC₆H₅ F.W. 212.25 CAS #579-44-2 m.p. 134-136°C

Benzoin Ethyl Ether

(ethoxy phenylacetophenone) C₆H₅COCH(CO₂H₅)C₆H₅ F.W. 240.31 CAS #574-09-4 m.p. 56-59°C

Benzoin Methyl Ether, UV Catalyst

[alpha-Ethoxy-alpha-phenylacetophenone) C₆H₅CH(OCH₃)COC₆H₅ F.W. 226.28 CAS #3524-62-7 Assay.>98% m.p. 47 - 49°C

(BDMA), Benzyldimethylamine

N-Benzyl-N, N-Dimethylamine C₆H₅CH₂N(CH₃)₂ FW. 135.21 CAS #103-83-3 b.p. 177-180 °C An amine accelerator for polymerization of epoxy resins. A direct alternative to DMP-30 with a longer shelf life and better penetration. Purity: 98%

TechnicalTip

Use of Accelerators for Epoxy Resins

Biebrich Scarlet, Water Soluble

(Bonceau BS; Acid Red 66; Croceine Scarlet; Double Scarlet BSF) C₂₂H₁₄N₄Na₂O₇S₂ F.W. 556.4 9 CAS #4196-99-0 Solubility: 5.0% Water; 0.25% Alcohol; 1.5% Cellosolve; 1.5% Glycol; 0.0% Xylene

BioBond; Tissue Section Adhesive

Please seelistinginAdhesives and Mountants.

Biomeda Mounting Media

Please seelistinginAdhesives and Mountants.

BioMount

Please seelistinginAdhesives and Mountants.

Bismarck Brown Y

(Basic Brown 1; Basic Brown G, GX, GXP; Vesuvine; Leather Brown; Manchester Brown; Phenylene Brown) C₁₈H₁₈N₈·2HCl F.W. 419.32 CAS #10114-58-6 Solubility: 1.5% Water; 3.0% Alcohol; 3.0% Cellosolve; 7.9% Glycol; 0.05% Xylene

Bismuth Carbonate, Basic, Purified

(Bismuth Subcarbonate) Approx. (BiO)₂CO₃ CAS # 5892-10-4 Used for staining synapses. Pfenninger, K., Sandri, C., Albert, K. and Eugster, C.H. (1969). Contribution of the problem of structural organization of the presynaptic area. Brain Res., 12:10

Bismuth Stain Kit

Bismuth specifically stains Golgi beads and interchromatin granules after fixation with glutaraldehyde, whereas it stains nucleoli, basic protein, and biogenic amines after formaldehyde fixation. Bismuth staining of sections is relatively nonspecific: it can stain glycogen, lysosomes, ribosomes, polysaccharides, ferritin, and DNA. M.A. Hayat, \"Basic Techniques for TEM\" p. 184 (1986).

1. Specific En Bloc Staining (Locke & Huie, 1977)

RT	11430	Bismuth Stain Kit consists of:	Kit	35.00	Add to Cart
10x5 ml Solution A (Sodium Tartrate, NaOH and Bismuth Subnitrate)
10x10 ml Solution B (.2M Triethanolamine-HCl buffer, pH 7.0)

2. Enhancing Contrast in General (Riva, 1974)

Kit contains: 5x10 ml Solution A(Sodium tartrate in NaOH) 5x200mg Ingredient B (Bismuth subnitrate)

3. Staining of Mucosubstances & Polysaccharides (Ainsworth et al., 1972)

Kit contains: 10x10 ml Solution A (Periodic acid, Ethanol, Sodium acetate) 10x10 ml Solution B (Sodium tartrate, NaOH, Bismuth subnitrate)

Bismuth Subnitrate, Crystalline

(Bismuth Nitrate, Basic; Bismuth Oxynitrate Hydrate) 4BiNO₃(OH₂)^.BiO(OH) F.W. 1461.99 CAS #1304-85-4 Bismuth staining for light and electron microscopy. M .Locke and P. Huie, Tissue & Cell, 9, 347 (1977); G.L. Brown & M. Locke, ibd. 10, 365 (1978)

Borax Tetra, Sodium Borate, Reagent, A.C.S.

(Sodium Tetraborate, Decahydrate) Na₂B₄O₇^.10H₂O FW. 381.37 CAS #1303-96-4 A.C.S. Specifications

Boric Acid, Reagent, Powder, A.C.S.

H₃BO₃ FW 61.83 CAS #10043-35-3 Assay 99.95% minimum. A buffer component in Tris-EDTA-Borate buffer. Also used in Silver-Methenamine buffer. Swift, J.A. (1968). The Electron histochemistry of cystine-containing proteins in thin transverse sections of human hair. J.R. Microsc. Soc. 88,449.

Bouin\'s Fixative

Please seelistingin theFixativessection.

Brilliant Cresyl Blue, Certified, C.N.#DcV-5

(Brilliant Blue C; Cresyl Blue 2RN) C₁₇H₂₁N₄OCl F.W. 332.84 CAS #10127-36-3 Solubility: 3.0% Water; 2.6% Alcohol; 2.25% Cellosolve; 8.0% Glycol; 9.5% Xylene

Bromophenol Blue, Reagent, A.C.S.

(3\',3\",5\' 5\"-Tetrabromophenol-sulfonthalein) C₁₉H₁₀O₅SBr₄ F.W. 669.97 CAS #115-39-9

Buffers; Prepared

Please see our complete listing ofPrepared Buffers.

1,4-Butanediol Diglycidyl Ether;

(Araldite RD-2) C₁₀H₁₈O₄ F.W. 202.2 CAS #2425-79-8 Approx. 70% Specific Gravity: 1.10

2-Butoxy Ethanol

(Butyl Cellosolve®; Ethylene Glycol Monobutyl Ether) CH₃(CH₂)₃OCH₂CH₂OH F.W. 118.18 CAS #111-76-2 Specific Gravity: 0.90 b.p. 169°C

Butvar B-98 Powder

CAS #63148-65-2 (Terpolymer of polyvinyl butyl, polyvinyl alcohol & polyvinyl acetate) Polyvinyl butyl resin for thin film support. Stronger than Formvar and more sticky, resulting in a firm attachment for slotted or meshed grids. The standard solution is 0.25% Butvar B-98 in Chloroform. Handley, D.A., et. al., Ultramicroscopy, 4,479 (1979).

Butvar B-98 Solution

Stock solution: 1.0% in Chloroform.

TechnicalTip

Tips for Butvar B-98 Support Films

n-Butyl Acetate, Reagent, A.C.S.

CH₃COO(CH₂)₃CH₃ F.W. 116.16 CAS #123-86-4 b.p . 127°C Specific Gravity: 0.88

n-Butyl Alcohol, Reagent, A.C.S.

(Butanol) CH₃CH₂CH₂CH₂OH F.W. 74.12 CAS # 71-36-3

TechnicalTip

Preservation of Phospholipids

(BGE), n-Butyl Glycidyl Ether, (Araldite RD-1)

(Butoxymethyl oxirane) C₇H₁₄O₂ F.W. 130.19 CAS #2426-08-6 b.p. 166-168°C Specific Gravity: 0.912

n-Butyl Methacrylate, Monomer

CH₂C(CH₃)CO₂CH₂CH₂CH₂CH₃ F.W. 142.20 CAS #97-88-1 b.p. 162-164°CStabilized with 10ppm MEHQ. (Hydroquinone, Mononethylether)